If reduce kasII enzyme to limit by using Hairpin-antisense the seed is aborted very soon and can not development

Two seeds can not development (left) next a normal seed (right) from a young silique of kasII-Hairpin antisense transgenic plant

Three aborted seeds in a mature silique of kasII-Hairpin antisense transgenic plant

Can see ratio 1(red, aborted):2(red, normal):1(non-red).

If T1 seeds with high suppression of Hairpin-antisense, T2 seeds in homozygous form could not development